Bacteria was categorised into two groups in by the Danish Bacteriologist Christian, gram positive and gram negative by a staining technique where the ability to avoid de-coloration of Crystal Violet solution by alcohol would render the category of gram positive, and gram negative if the bacteria is de-coloured. This could be noted by the final colour of the bacteria:
Order now Bacteria that are non-responsive to this technique are known as gram insensitive.
I hypothesize that in the cultures used in this lab for gram staining will contain many gram negative and gram positive bacteria. Material and Methods The following things are needed to run a gram staining experiment: Bunsen Burner Lighter will suffice Prepare and heat-fix smears.
Stain the slides as follows: Flood the crystal violet for one minute. Pour off excess dye and wash gently in tap water and drain the slide against a paper towel. Wash with tap water and drain carefully.
Wash with tap water at the end of the 30 seconds to stop the decentralization. Wash, drain, blot, and examine under oil.
Draw the cells showing morphology, grouping, and relative sizes. Color a few of the cells of each bacterial species to show the Gram reaction. Looking under the microscope after letting the stain set will prove to be quite interesting. Results The gram staining lab was a huge success.
Many different types of both gram negative and gram positive bacteria were identified. There were even a few gram insensitive fellows that had some interesting characteristics such as being spiral shaped.Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining.
Gram Stains is used to identify and classify bacteria, it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative.
The foloowing is a breif summerary of the gram staining procedure. Gram staining is a bacteriological laboratory technique used to differentiate bacterial species into two large groups (Gram-positive and Gram-negative) based on the physical properties of their cell walls..
Gram staining is the single most important and universally used staining technique in the laboratory. The name comes from it’s inventor Hans Christian Gram who developed the technique in (published ).
Gram staining is almost always the ﬁrst step in identifying a bacteria. The process is. Gram staining is a differential or specific method of staining.
Gram staining is a way to tell the difference between gram positive and gram negative bacteria.
The cell wall of the gram positive bacteria is made up of several “layers of peptidoglygan,” and “techoic acids,” (LabPaq, p. ). The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative.
This gram stain technique was discovered by Hans Christina Gram in Gram Staining Essay. Title: Identification of Two Unknown Bacteria’s Through the Use of Gram Staining Introduction: The Gram staining of bacteria is a widely used method to identify unknown bacteria.
The purpose of this method is to find out identities of unidentified bacteria.